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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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PE-Labeled CD19 His Tag Protein, Human

CD19,B4,CVID3,MGC12802

价格 ￥2,320.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011320
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    CD19,B4,CVID3,MGC12802

  • Accession

    P15391-1

  • 表达序列

    Pro 20-Lys 291 with His Tag at C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    40-45kDa (Reducing)

  • 标记

    PE

  • 标签

    His Tag

  • 性状

    Liquid

  • 缓冲体系

    PBS 0.5%BSA pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Wang SY, An WH, Wang ZS, Wang WL, Zhang B, Xu KL, Guo SL, Gao M, Li B, Huang L, Tian HH, Guo WY, Wang HR. Incidentally cured psoriasis in a patient with refractory/relapsed diffuse large B-cell lymphoma receiving CD19 CAR-T cell therapy: a case report. Front Immunol. 2024 Oct 24; 15: 1418768.

背景介绍

  • CD19, also known as B-lymphocyte antigen or Cluster of Differentiation 19, is a single-pass type I membrane protein that contains two Ig-like C2-type (immunoglobulin-like) domains. It is expressed on follicular dendritic cells and B cells, being present from the earliest recognizable B-lineage cells during development to B-cell blasts, but is lost upon maturation to plasma cells. CD19 primarily functions as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, leading to binding by Src-family kinases and recruitment of PI-3 kinase. Mutations in the CD19 gene are associated with severe immunodeficiency syndromes, characterized by diminished antibody production. CD19 has been shown to interact with CD81, CD82, Complement receptor 2, and VAV2.

注意事项

  • 请严格按照推荐用量进行实验，分群不明显情况下，可适当提高2-5倍推荐用量。

  • 流式分析

    • 2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with 0.5ug PE-Labeled CD19 His Tag Protein, Human, (Cat. No. UA011320) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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