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物种
Human分子别名
IL27RA,CRL1,WSX1,TCCR,IL-27R-alpha,ZcytoR1Accession
Q6UWB1表达序列
Gly34-Lys516 with Fc at the C-Terminus
表达宿主
HEK293分子量
100-120kDa (Reducing)纯度
＞95% by SDS-PAGE & > 90% SEC-HPLC活性
Immobilized IL-27 Protein, Human (Cat. No. UA040457) at 2.0μg/mL (100μL/well) can bind IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human (Cat. No. UA011319) with EC50 of 10.03-15.25 ng/mL.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Poole JA, Schwab A, Thiele GM, England BR, Nelson AJ, Gleason A, Duryee MJ, Bailey KL, Romberger DJ, Hershberger D, Van De Graaff J, May SM, Walenz R, Kramer B, Mikuls TR. Unique transcriptomic profile of peripheral blood monocytes in rheumatoid arthritis-associated interstitial lung disease. Rheumatology (Oxford).
2.Yang Y, Chen Y, Zhang L, Yang X, Qiao J. Factors influencing the development of osteoporosis in elderly patients with rheumatoid arthritis. Afr Health Sci. 2024 Dec;24(4):308-312.
The interleukin-27 receptor subunit alpha (IL27RA), also known as WSX-1 or TCCR, is a member of the type I cytokine receptor family that forms the interleukin-27 receptor heterodimer together with glycoprotein 130 (IL6ST/gp130). Structurally characterized by three fibronectin type-III domains, IL27RA is highly expressed in lymphoid tissues including spleen, lymph nodes, and peripheral blood leukocytes, with particularly strong expression in resting/naive CD4+ and CD8+ T cells.Functioning as the specific ligand-binding component for interleukin-27, IL27RA requires association with gp130 for effective signal transduction through STAT1 and STAT3 pathways. This receptor system plays crucial roles in both adaptive and innate immunity. In adaptive immunity, it promotes Th1-type immune responses by initiating Th1 cell differentiation through STAT1 activation and T-bet expression induction, while also stimulating proliferation of naive CD4+ T cells. In innate immunity, IL27RA contributes to innate defense mechanisms through direct actions on cells of the innate immune system. The receptor shares structural and sequential homology with the IL-12 receptor β2 chain, placing it within the IL-6/IL-12 cytokine family, and serves as a key regulator in bridging innate and adaptive immune responses.
电泳
2μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human is more than 90% determined by SEC-HPLC.
ELISA
Immobilized IL-27 Protein, Human (Cat. No. UA040457) at 2.0μg/mL (100μL/well) can bind IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, Human (Cat. No. UA011319) with EC50 of 10.03-15.25 ng/mL.
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