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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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FITC-Labeled GUCY2C Fc Chimera Protein, Human

GUC2C,STAR,STA receptor,hSTAR,GC-C,DIAR6

价格 ￥2,320.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011281
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    GUC2C, STAR, STA receptor, hSTAR, GC-C, DIAR6

  • Accession

    P25092

  • 表达序列

    Ser24-Gln430 with Human IgG1 Fc Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    95-120kDa

  • 标记

    FITC

  • 标签

    Human IgG1 Fc

  • 性状

    Liquid

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Michael S. Magee,Crystal L. Kraft,Tara S. Abraham,Trevor R. Baybutt,Glen P. Marszalowicz,Peng Li: GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity. OncoImmunology, Volume 5, 2016 - Issue 10.

背景介绍

  • Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. Endogenous ligands of GUCY2C include guanylin and uroguanylin. GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior. The activation of cyclic guanosine monophosphate (cGMP), a second intracellular messenger, can stimulate the production of cyclic guanosine monophosphate (CGMP), which plays a role in regulating homeostasis, maintaining intestinal barrier function, and exerting anti-inflammatory activity.

注意事项

  • 请严格按照推荐用量进行实验，分群不明显情况下，可适当提高2-5倍推荐用量。

  • 流式分析

    • 2e5 of transient transfected anti- GUCY2C ScFv CAR-293 cells were stained with 0.1ug FITC-Labeled GUCY2C Fc Chimera Protein, Human, (Cat. No. UA011281) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- GUCY2C ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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