ACHE/Acetylcholinesterase His Tag Protein, Human

ARACHE,apoptosis-related acetylcholinesterase,N-ACHE

价格￥2,480.00 （供应商现货： 3-5个工作日）

货号 UA011275

规格

25μg100μg500μg

数量

产品介绍

产品介绍评论(0)

产品规格

物种

Human
分子别名

ARACHE,apoptosis-related acetylcholinesterase,N-ACHE
Accession

P22303-1
表达序列

Glu32-Leu614 with His Tag at the C-Terminus
表达宿主

HEK293
分子量

65-80kDa (Reducing)
纯度

＞90% by SDS-PAGE
活性

Measured by its ability to cleave Acetylthiocholine. The specific activity is >1000 nmol/min/μg, as measured under the described conditions.
标记

Unconjugated
标签

His Tag
性状

Lyophilized Powder
缓冲体系

PBS, PH7.4, 5% trehalose
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1.Rawat K, Tewari D, Bisht A, Chandra S, Tiruneh YK, Hassan HM, Al-Emam A, Sindi ER, Al-Dies AM. Identification of AChE targeted therapeutic compounds for Alzheimer's disease: an in-silico study with DFT integration. Sci Rep. 2024 Dec 5;14(1):30356.
2.Reynoso-García MF, Nicolás-Álvarez DE, Tenorio-Barajas AY, Reyes-Chaparro A. Structural Bioinformatics Applied to Acetylcholinesterase Enzyme Inhibition. Int J Mol Sci. 2025 Apr 17;26(8):3781.

背景介绍

Acetylcholinesterase (AChE), also known as acetylhydrolase, is a crucial serine hydrolase that efficiently terminates signal transmission at neuromuscular junctions and cholinergic synapses by rapidly hydrolyzing acetylcholine (ACh) in the synaptic cleft. Beyond its fundamental role in neurotransmitter regulation, AChE has been found to participate in neuronal apoptosis and other cellular processes, suggesting a broader involvement in programmed cell death. In neurodegenerative diseases such as Alzheimer's disease, acetylcholine deficiency is closely associated with cognitive decline, making AChE an important therapeutic target. Currently, acetylcholinesterase inhibitors (AChEIs) are widely used in clinical practice for the targeted treatment of Alzheimer's disease and other forms of dementia.

操作步骤

实验方法
实验原理：检测ACHE蛋白切割Acetylthiocholine的能力。
实验材料
1. 检测缓冲液：0.1 M Sodium Phosphate, 0.05% (w/v) Brij-35,pH 7.5
2. ACHE/Acetylcholinesterase His Tag Protein, Human (SPA1701)
3. 底物：Acetylthiocholine (ATC) (A5626)
4. 5’,5’-Dithiobis (2-nitrobenzoic acid) (DTNB) (D8130)
5. 96 Well Clear Plat (701311)
6. 读板器（PerkinElmer，405 nm）
实验步骤
1. 在检测缓冲液中，将ACHE蛋白稀释至 0.02 μg/mL。
2. 在含有100 μM DTNB的检测缓冲液中，将底物稀释至200 μM。
3. 向透明板中加入50 μL稀释后的ACHE蛋白。同时设置一个以检测缓冲液代替 ACHE protein的对照组。
4. 加入 50 μL 底物/DTNB 混合液以启动反应。
5. 在 405 nm 吸光度下，以动力学模式读取信号10分钟。
6. 计算特异性活性：
Specific Activity (pmol/min/µg) =
Adjusted Vmax* (OD/min) x well volume (L) x 10⁹ nmol/mol
ext. coeff** (M^--1cm^--1) x path corr.*** (cm)
x amount of enzyme (μg)
*底物空白校正
**使用消光系数 13260 M⁻¹cm⁻¹
***使用光程校正 0.32 cm