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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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Alexa Fluor 647-Labeled B7-H3/CD276 Fc Chimera Protein, Human

B7 homolog 3 (B7-H3),CD276,B7 homolog 3

价格 ￥2,320.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011269
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    B7 homolog 3 (B7-H3), CD276, B7 homolog 3

  • Accession

    Q5ZPR3-2

  • 表达序列

    Leu29-Pro245 with Human IgG1 Fc at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    60-70kDa

  • 标记

    Alexa Fluor® 647

  • 标签

    Human IgG1 Fc

  • 性状

    Liquid

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. A I Chapoval et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.Nat Immunol. 2001 Mar;2(3):269-74.

背景介绍

  • B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain. Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response. Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation. One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature.

  • 流式分析

    • 2e5 of transient transfected anti- CD276 ScFv CAR-293 cells were stained with 0.01ug Alexa Fluor 647-Labeled B7-H3/CD276 Fc Chimera Protein, Human, (Cat. No. UA011269) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- CD276 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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