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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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FITC-Labeled MSLN/Mesothelin Fc Chimera Protein, Human

MSLN/Mesothelin,MPF,SMRP

价格 ￥2,320.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011264
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    MSLN/Mesothelin, MPF, SMRP

  • Accession

    Q13421-1

  • 表达序列

    Glu296-Gly580, with C-hIgG1 Fc

  • 表达宿主

    HEK293

  • 分子量

    70-85kDa

  • 标记

    FITC

  • 标签

    Human IgG1 Fc

  • 性状

    Liquid

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Chang K. et al. (1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. PNAS. 93(1): 136-140.
    2、Liu X. et al. (2020) Multiple proteases are involved in mesothelin shedding by cancer cells. Commun Biol. 3: 728.

背景介绍

  • Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte potentiating factor, which belongs to the mesothelin family. Mesothelin/MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin/MSLN can interacts with MUC16. The membrane-anchored form of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro. Mesothelin (MSLN) is a lineage restricted cell surface protein expressed in about 30% of human cancers and high MSLN expression is associated with poor survival in several different cancers. The restricted expression of MSLN in normal tissue and its frequent expression in cancers make MSLN an excellent target for antibody-based therapies.

  • 流式分析

    • 2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with 0.1ug FITC-Labeled MSLN/Mesothelin Fc Chimera Protein, Human, (Cat. No. UA011264) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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