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产品中心 药物靶点和Fc受体 药物靶点

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GFRAL Fc Chimera Protein, Human

GFR alpha like,GRAL,GDNF family receptor alpha-like

价格 ￥1,280.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011250
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    GFR alpha like,GRAL,GDNF family receptor alpha-like

  • Accession

    Q6UXV0

  • 表达序列

    Ser19-Glu351 with Human IgG1 Fc at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    60-80kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE &SEC-HPLC

  • 活性

    Immobilized GDF-15/MIC-1 Protein, Human (Cat. No. UA040137) at 2.0μg/mL (100μL/well) can bind GFRAL Fc Chimera Protein, Human with EC50 of 3.84-4.89 ng/mL. Immobilized GDF-15 Protein, Mouse (Cat. No.UA040185) at 2.0μg/mL (100μL/well) can bind GFRAL Fc Chimera Protein, Human (UA011250)with EC50 of 11.85-19.23 ng/mL.

  • 标记

    Unconjugated

  • 标签

    Human IgG1 Fc

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Airaksinen MS, Holm L, Hätinen T. Evolution of the GDNF family ligands and receptors. Brain Behav Evol. 2006;68(3):181-90.
    2.Emmerson PJ, Wang F, Du Y, Liu Q, Pickard RT, Gonciarz MD, Coskun T, Hamang MJ, Sindelar DK, Ballman KK, Foltz LA, Muppidi A, Alsina-Fernandez J, Barnard GC, Tang JX, Liu X, Mao X, Siegel R, Sloan JH, Mitchell PJ, Zhang BB, Gimeno RE, Shan B, Wu X. The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL. Nat Med. 2017 Oct;23(10):1215-1219.

背景介绍

  • GFR alpha-like is also known as GDNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356, PRO34128. Macrophage inhibitory cytokine-1/growth differentiation factor 15 (MIC-1/GDF15) is a divergent transforming growth factor (TGFbeta) superfamily cytokine implicated in biological and disease processes including metabolism, cancer, and chronic inflammation. GDNF family receptor α-like (GFRAL) was recently identified as the neuronal brainstem receptor responsible for mediating the anorectic actions of GDF15. Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses . Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of GFRAL Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.

  • ELISA

    • Immobilized GDF-15/MIC-1 Protein, Human (Cat. No. UA040137) at 2.0μg/mL (100μL/well) can bind GFRAL Fc Chimera Protein, Human with EC50 of 3.84-4.89 ng/mL.

    • Immobilized GDF-15 Protein, Mouse (Cat. No.UA040185)
      at 2.0μg/mL (100μL/well) can bind GFRAL Fc Chimera
      Protein, Human (UA011250)with EC50 of 11.85-19.23
      ng/mL.

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