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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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Alexa Fluor 647-Labeled NKG2D/CD314 Fc Chimera Protein, Human

Killer cell lectin-like receptor subfamily K member 1,NK cell receptor D,NKG2-D-activating NK receptor,CD314,D12S2489E,KLR,DNA Segment On Chromosome 12 (Unique) 2489 Expressed Sequence,Killer Cell Lectin Like Receptor K1,NKG2-D,Killer Cell Lectin-Like Receptor Subfamily K,Member 1,KLRK1,CD314 Antigen

价格 ￥7,450.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011245
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Killer cell lectin-like receptor subfamily K member 1，NK cell receptor D，NKG2-D-activating NK receptor，CD314，D12S2489E，KLR，DNA Segment On Chromosome 12 (Unique) 2489 Expressed Sequence，Killer Cell Lectin Like Receptor K1，NKG2-D，Killer Cell Lectin-Like Receptor Subfamily K， Member 1，KLRK1，CD314 Antigen

  • Accession

    P26718-1

  • 表达序列

    Phe78-Val216 with MD at the N-Terminus and Human IgG1 Fc at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    50-70kDa (Reducing)

  • 标记

    Alexa Fluor® 647

  • 标签

    Human IgG1 Fc

  • 性状

    Liquid

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. 2.Diefenbach, A. et al. (2001) Nature 413:165. 3.NKG2D and its Ligands (2002) www.rndsystems.com

背景介绍

  • NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

  • 流式分析

    • 2e5 of transient transfected anti-NKG2D/CD314 ScFv CAR-293 cells were stained with 0.01ug Alexa Fluor 647-Labeled NKG2D/CD314 Fc Chimera Protein, Human, (Cat. No. UA011245) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity.
      2e5 of transient transfected anti-NKG2D/CD314 ScFv CAR-293 cells were stained with 0.01ug NKG2D/CD314 Fc Chimera Protein, Human (Cat. No. UA010732) (Fig. D). Alexa Fluor 647 signal was used to evaluate the binding activity.
      2e5 of transient transfected anti-NKG2D/CD314 ScFv CAR-293 cells were stained with isotype and G4S Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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