Siglec-3 (R69G) His Tag Protein, Human
Myeloid Cell Surface Antigen CD33,CD33 Molecule Transcript,CD33 Antigen,Siglec-3,P67,FLJ00391,Sialic acid-binding Ig-like lectin 3,CD33,CD33 Molecule,CD33 Antigen (Gp67),SIGLEC3,Gp67,Sialic Acid Binding Ig-Like Lectin 3
分享产品介绍 评论(0)
物种
Human分子别名
Myeloid Cell Surface Antigen CD33,CD33 Molecule Transcript,CD33 Antigen,Siglec-3,P67,FLJ00391,Sialic acid-binding Ig-like lectin 3,CD33,CD33 Molecule,CD33 Antigen (Gp67),SIGLEC3,Gp67,Sialic Acid Binding Ig-Like Lectin 3Accession
P20138表达序列
Asp18-His259 (Arg69Gly) with His Tag at C-Terminus
表达宿主
HEK293分子量
43-55kDa (Reducing)
纯度
>95% as determined by SDS-PAGE & SEC-HPLC内毒素含量
<0.1EU/μg活性
Immobilized Siglec-3 (R69G) His Tag Protein, Human (Cat. No. UA011232) at 2.0μg/mL (100μL/well) can bind Anti-Human SIGLEC3 (Gemtuzumab) with EC50 of 2.64-4.99ng/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Ulyanova T. et al. (1999) The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 29(11): 3440-9.
2、Freeman S D. et al. (1995) Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood. 85(8): 2005-12.
Siglecs are transmembrane proteins of the immunoglobulin superfamily that bind sialic acid. They include an N-terminal V-set Ig-like domain and various C2-set domains. The first characterized Siglecs, including Siglec-1 to -4, show 25–30% sequence identity in their extracellular regions. A cluster of Siglec genes related to CD33 is found on human chromosome 19q13.3-4. The CD33-related subgroup (Siglecs-3, -5, -6, -7, and -8) shares 50–70% sequence identity. Each Siglec has a unique role in hemopoietic cell biology.
CD33 (Siglec-3), the smallest Siglec member, binds α2-6- and α2-3-sialylated glycans and is highly expressed on leukemic cell lines. It's primarily found on myeloid lineage leukocytes but also appears on lymphoid cells, including NK cells. CD33 exists as two membrane isoforms: full-length CD33M and CD33m, which lacks the V extracellular domain. CD33 expression is linked to Alzheimer’s disease, autoimmune conditions like SLE, type II diabetes, and infections.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of Siglec-3 (R69G) His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized Siglec-3 (R69G) His Tag Protein, Human (Cat. No. UA011232) at 2.0μg/mL (100μL/well) can bind Anti-Human SIGLEC3 (Gemtuzumab) with EC50 of 2.64-4.99ng/mL.
评论(0)