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产品中心 药物靶点和Fc受体 CAR-T荧光标记靶点蛋白

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Alexa Fluor 647-Labeled CD19 Fc Chimera Protein, Human

B4,CVID3

价格 ￥2,320.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011229
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    B4; CVID3

  • Accession

    P15391-1

  • 表达序列

    Pro 20-Lys 291 with Human IgG1 Fc at the C-Terminus

  • 表达宿主

    CHO

  • 分子量

    73-88kDa (Reducing)

  • 标记

    Alexa Fluor® 647

  • 标签

    Human IgG1 Fc

  • 性状

    Liquid

  • 缓冲体系

    PBS, pH7.4

  • 储存条件

    · 12 months from date of receipt stored at --20 to -80℃.

    · 1 week, 2 to 8℃ under sterile conditions

    · Recommend to aliquot the protein into smaller quantities for optimal storage.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
    Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
    Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
    Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.

背景介绍

  • CD19 is a 95 kDa type-I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is extensively expressed on B cells during most stages of B-cell differentiation, with expression levels decreasing as B cells terminally differentiate into plasma cells. Genetic mapping places CD19 at the 16p11.2 locus on chromosome 16, where it encodes a 540 amino acid protein. This protein features two extracellular C-type IgSF domains and a large cytoplasmic tail of approximately 240 residues, which shows significant conservation between mice and humans.
    CD19 functions as a signal-amplifying coreceptor, with its expression limited to B cells and follicular dendritic cells. It is part of a multimolecular complex on the B cell surface that includes CD21 (complement receptor type 2, CR2), the tetraspanin CD81, and CD225. Through its interaction with CD21, CD19 acts as a signal transducing component for antigens bound by complement. The co-ligation of the B cell receptor (BCR) and the CD19/CD21 complex significantly lowers the activation threshold of B cells by approximately two orders of magnitude.
    Recognizing its role in B cell biology, CD19 has emerged as a potential target for monoclonal antibody therapy. Early data have shown its potential in B-cell depletion, presenting an attractive treatment option for autoimmune diseases and malignant B-cell lymphomas. The rationale for CD19 as a target for immunotherapy includes: (1) its expression on the majority of B-cell malignancies; (2) its early expression in the B-cell lineage, preceding even CD20; (3) its efficient internalization in lymphoma tumor models; and (4) its potential involvement in the development of B-cell cancers.

  • 流式分析

    • 2e5 of transient transfected anti-CD19 ScFv CAR-293 cells were stained with 0.01ug Alexa Fluor 647 Labeled- CD19 Fc Chimera Protein, Human, (Cat. No. UA011229) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with 0.01ug CD19 Fc Chimera Protein, Human (Cat. No. UA010042) (Fig. D). APC signal was used to evaluate the binding activity.
      2e5 of transient transfected anti- CD19 ScFv CAR-293 cells were stained with isotype and G4S Linker-Alexa Fluor® 488 (Fig. E and F). Alexa Fluor® 488 signal was used to evaluate the binding activity.

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