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物种
Human分子别名
VAV1Accession
P15498-1浓度
1mg/ml by Nanodrop表达序列
Lys782-Cys845, with GST tag at N-terminal
表达宿主
E.coli分子量
33-43kDa (Reducing)
纯度
＞90% by SDS-PAGE & SEC-HPLC内毒素含量
<0.1EU/μg活性
The VAV1 activity was assayed by HTRF technology. The reaction was performed by incubating the VAV1 protein and CRBN, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.标记
Unconjugated标签
GST Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
·Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
文献引用
1. Chrencik J E, Brooun A, Zhang H, et al. Structural basis of guanine nucleotide exchange mediated by the T-cell essential Vav1[J]. Journal of molecular biology, 2008, 380(5): 828-843.
Vav1 Guanine Nucleotide Exchange Factor (VAV1) is a member of the VAV gene family and is exclusively expressed in the hematopoietic system. It functions as a GDP/GTP nucleotide exchange factor (GEF), activating pathways that lead to actin cytoskeletal rearrangements and transcriptional alterations. VAV1 contains several characteristic structural motifs that are crucial for its role as a versatile signal transducer.
VAV1 is essential for hematopoiesis, playing a key role in the development and activation of T cells and B cells. It has also been identified as a specific binding partner for Nef proteins from HIV-1.
Expression of VAV1 is restricted to cells of hematopoietic origin, including those of the erythroid, lymphoid, and myeloid lineages. Diseases associated with VAV1 include cherubism and HIV-1 infection.
生物活性
The VAV1 activity was assayed by HTRF technology. The reaction was performed by incubating the VAV1 protein and CRBN, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of VAV1 Protein Human is greater than 90% as determined by SEC-HPLC.
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