Cathepsin F His Tag Protein, Human

Cathepsin F,CTSF

价格￥4,200.00 （供应商现货： 3-5个工作日）

货号 UA011221

规格

100μg25μg500μg

数量

产品介绍

产品介绍评论(0)

产品规格

物种

Human
分子别名

Cathepsin F,CTSF
Accession

Q9UBX1
表达序列

Ala20-Asp484 with His Tag at the C-Terminus
表达宿主

HEK293
分子量

60-75kDa (Reducing)
纯度

＞95% by SDS-PAGE
活性

The specific activity is >10 pmol/min/μg, as measured under the described conditions.
标记

Unconjugated
标签

His Tag
性状

Lyophilized Powder
缓冲体系

PBS, PH7.4, 5% trehalose
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1.Wang X, Zhu Z. Unraveling the causal relationship and underlying mechanisms between cathepsins on liver cancer: findings from mendelian randomization and bioinformatics analysis. Discov Oncol. 2025 Mar 7;16(1):277.
2.Mohan S, Sampognaro PJ, Argouarch AR, Maynard JC, Welch M, Patwardhan A, Courtney EC, Zhang J, Mason A, Li KH, Huang EJ, Seeley WW, Miller BL, Burlingame A, Jacobson MP, Kao AW. Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration. Mol Neurodegener.

背景介绍

CTSF (Cathepsin F) is a cysteine protease primarily localized in lysosomes. Its protein structure contains a typical catalytic domain of cysteine proteases, which specifically cleaves peptide bonds through its active site. Within the cell, it is responsible for degrading abnormal or damaged proteins and participates in antigen processing and presentation, thereby regulating immune responses. Clinically, aberrant expression or dysfunction of CTSF is closely associated with various diseases: its overexpression may promote tumor invasion and metastasis, while in autoimmune diseases, it may lead to immune imbalance due to disrupted antigen processing. Consequently, CTSF has emerged as a potential disease biomarker and therapeutic target.

操作步骤

实验方法
实验原理：检测Cathepsin F His Tag Protein, Human切割荧光肽底物Z-FR-AMC的能力。
实验材料
蛋白激活缓冲液：50 mM sodium acetate pH 4.5, 5 mM DTT
检测缓冲液：100 mM Sodium acetate (pH 5.5), 2 mM EDTA, 8 mM DTT
Cathepsin F His Tag Protein, Human (UA011221)
底物：Z-FR-AMC (C350145)
96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
读板器（PerkinElmer，激发波长360 nm，发射波长460 nm）

实验步骤
Cathepsin F His Tag Protein, Human在蛋白激活缓冲液中于室温下激活90分钟，然后进行反应。
在检测缓冲液中将激活的Cathepsin F His Tag Protein, Human稀释至15 µg/mL、7.5 µg/mL、3.75 µg/mL、1.875 µg/mL、0.93575 µg/mL、0.48 µg/mL、0 µg/mL。
在检测缓冲液中将Z-FR-AMC稀释至40 µM。
向黑色孔板中加入稀释后的Cathepsin F His Tag Protein, Human，每孔50 µL，然后加入50 µL的40 µM底物，30°C孵育40-120 min。
在激发和发射波长分别为360 nm和460 nm的情况下读取荧光信号。
计算特异性活性：
Specific Activity (pmol/min/µg) = Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU)
Incubation time (min) x amount of enzyme (µg)
*底物空白校正
**使用校准标准品7-氨基-4-甲基香豆素（7-amino-4-Methyl Coumarin）推导