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产品中心 药物靶点和Fc受体 药物靶点

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SIRP alphaV2/CD172a Fc Chimera Protein, Human

CD172 antigen-like family member A,CD172a antigen,CD172a

价格 ￥800.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011151
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    CD172 antigen-like family member A, CD172a antigen, CD172a

  • Accession

    ATD50864.1

  • 表达序列

    Glu31-Arg369 with Human IgG1 Fc at C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    75-90kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & SEC-HPLC

  • 活性

    Immobilized CD47 His Tag Protein, Human (Cat. No. UA010014) at 2.0μg/mL (100μL/well) can bind SIRP alphaV2/CD172a Fc Chimera Protein, Human (Cat. No. UA011151) with EC50 of 10.50-16.63ng/mL.

  • 标记

    Unconjugated

  • 标签

    Human IgG1 Fc

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Ide, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5062.
    2.Gardai, S.J. et al. (2003) Cell 115:13.
    3.Lundberg, P. et al. (2007) Biochem. Biophys. Res. Commun. 352:444.

背景介绍

  • Signal regulatory protein alpha (SIRP alpha, CD172a), also known as SHPS-1 and formerly MyD-1, is a ~90 kDa type I transmembrane glycoprotein in the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPs are paired receptors with similar extracellular domains but distinct C-termini and functions. The human SIRP alpha, consisting of 503 amino acids, includes a 342 amino acid extracellular domain with one V-type and two C1 type Ig domains, and three potential N-glycosylation sites. It features a 110 amino acid cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 upon phosphorylation. SIRP alpha exhibits over 40 polymorphisms, including the notable BIT (also known as SIRP alpha 2 or PTPNS). Engagement of SIRP alpha generally elicits a negative regulatory signal. It recognizes CD47 on aged erythrocytes, platelets, or xenogenic cells, promoting their clearance from circulation. In the lung, SIRP alpha's recognition of surfactants SP-A and SP-D can inhibit alveolar macrophage cytokine production. Additionally, the CD47-SIRP alpha interaction facilitates macrophage fusion during osteoclastogenesis.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of SIRP alphaV2/CD172a Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.

  • ELISA

    • Immobilized CD47 His Tag Protein, Human (Cat. No. UA010014) at 2.0μg/mL (100μL/well) can bind SIRP alphaV2/CD172a Fc Chimera Protein, Human (Cat. No. UA011151) with EC50 of 10.50-16.63ng/mL.

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