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物种
Human分子别名
CD166 antigen,Activated leukocyte cell adhesion molecule,CD166,ALCAM,MEMD,Activated Leucocyte Cell Adhesion Molecule,Activated-Leukocyte Cell Adhesion MoleculeAccession
Q13740-1表达序列
Trp28-Ala526 with Human IgG1 Fc at C-Terminus表达宿主
HEK293分子量
90-120kDa (Reducing)
纯度
＞95% by SDS-PAGE &＞90% by SEC-HPLC内毒素含量
<0.1EU/μg活性
Anti-His antibody Immobilized on CM5 Chip captured Human CD6, His tag (Cat. No. S0A1037), can bind ALCAM/CD166 Fc Chimera Protein, Human (Cat. No. UA011127) with an affinity constant of 1.31 nM as determined in SPR assay.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.
Activated Leukocyte Cell Adhesion Molecule (ALCAM), also known as Cluster of Differentiation 166 (CD166), is a type I transmembrane cell adhesion molecule belonging to the immunoglobulin (Ig) superfamily. It acts as a ligand for CD6 and is expressed on T lymphocytes. The interaction between ALCAM and CD6 is essential for T cell development, regulation, and for the binding of T- and B-cells to activated leukocytes.
ALCAM is expressed in various tissues and is typically restricted to cell subsets involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response, and tumor progression. High expression levels of ALCAM on melanoma cell lines appear to promote metastasis, while in breast cancer, it has been associated with anti-metastatic activity. Additionally, ALCAM may influence the expression or adhesion of the neuronal adhesion molecule NCAM-L1, as observed in both the developing retina and invasive melanoma.
电泳
体积排阻色谱(SEC-HPLC)
ELISA
Immobilized ALCAM/CD166 Fc Chimera Protein, Human (Cat. No. UA011127) at 2.0μg/mL (100μL/well) can bind Biotinylated Human CD6, hFc tag with EC50 of 58.06-74.60 ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured Human CD6, His tag (Cat. No. S0A1037), can bind ALCAM/CD166 Fc Chimera Protein, Human (Cat. No. UA011127) with an affinity constant of 1.31 nM as determined in SPR assay.
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