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物种
Human分子别名
CD166 antigen，Activated leukocyte cell adhesion molecule，CD166，ALCAM，MEMD，Activated Leucocyte Cell Adhesion Molecule，Activated-Leukocyte Cell Adhesion MoleculeAccession
Q13740-1表达序列
Trp28-Ala526 with His Tag at C-Terminus
表达宿主
HEK293分子量
70-90kDa (Reducing)
纯度
＞95% by SDS-PAGE &＞90% by SEC-HPLC内毒素含量
<0.1EU/μg活性
Immobilized ALCAM/CD166 His Tag Protein, Human (Cat. No. UA011112) at 5 μg/mL (100μL/well) can bind Human CD6, hFc tag (Cat. No. S0A1088) with EC50 of 0.14-0.18 μg/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.
Activated Leukocyte Cell Adhesion Molecule (ALCAM), also known as Cluster of Differentiation 166 (CD166), is a type I transmembrane cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily. It serves as a ligand for CD6 and is expressed on T lymphocytes. The interaction between ALCAM and CD6 is crucial for T cell development, regulation, and for the binding of T- and B-cells to activated leukocytes.
ALCAM is expressed in a variety of tissues but is typically restricted to cell subsets involved in dynamic growth and/or migration. This includes processes such as neural development, branching organ development, hematopoiesis, immune response, and tumor progression. High expression levels of ALCAM on melanoma cell lines seem to promote metastasis, whereas in breast cancer, it has been associated with anti-metastatic activity.
ALCAM may also influence the expression or adhesion of the neuronal adhesion molecule NCAM-L1, as observed in both the developing retina and invasive melanoma.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of ALCAM/CD166 His Tag Protein, Human is greater than 90% as determined by SEC-HPLC.
ELISA
Immobilized ALCAM/CD166 His Tag Protein, Human (Cat. No. UA011112) at 5 μg/mL (100μL/well) can bind Human CD6, hFc tag (Cat. No. S0A1088) with EC50 of 0.14-0.18 μg/mL.
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