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产品中心 药物靶点和Fc受体 药物靶点

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GFRA1 Fc Chimera Protein, Human

GDNFRA,RETL1,TRNR1,GDNFR,GFR-ALPHA-1,RET1L

价格 ￥1,000.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011102
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L

  • Accession

    P56159-2

  • 表达序列

    Asp25-Ser424 with Human IgG1 Fc Tag at C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    75-90kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & ＞90% by SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    Immobilized GDNF Protein, Human (Cat. No. UA040211) at 1.0μg/mL (100μL/well) can bind GFRA1 Fc Chimera Protein, Human (Cat. No. UA011102) with EC50 of 5.75-7.71ng/mL.

  • 标记

    Unconjugated

  • 标签

    Human IgG1 Fc

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Mihwa Kim. GFRA1: A Novel Molecular Target for the Prevention of Osteosarcoma Chemoresistance. Int J Mol Sci. 2018 Apr 4;19(4):1078.

背景介绍

  • The Glycosylphosphatidylinositol-linked Glial Cell Derived Neurotrophic Factor (GDNF) receptor alpha (GFRA) is a coreceptor that recognizes the GDNF family of ligands and plays a crucial role in the development and maintenance of the nervous system. Among the four identified GFRA isoforms, GFRA1 specifically binds to GDNF and is involved in the regulation of neuronal cell proliferation, differentiation, and migration. Additionally, GFRA1 has been implicated in cancer cell progression and metastasis.
    Recent research indicates that GFRA1 can contribute to the development of chemoresistance in osteosarcoma. GFRA1 expression is induced following treatment of osteosarcoma cells with the widely used anticancer drug, cisplatin. This induction of GFRA1 expression significantly suppresses apoptosis mediated by cisplatin in osteosarcoma cells. Furthermore, GFRA1 expression promotes autophagy by activating the SRC-AMPK signaling axis after cisplatin treatment, leading to enhanced survival of osteosarcoma cells.
    GFRA1-induced autophagy has been shown to promote tumor growth in mouse xenograft models, suggesting a novel function for GFRA1 in osteosarcoma chemoresistance.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of GFRA1 Fc Chimera Protein, Human is greater than 90% as determined by SEC-HPLC.

  • ELISA

    • Immobilized GDNF Protein, Human (Cat. No. UA040211) at 1.0μg/mL (100μL/well) can bind GFRA1 Fc Chimera Protein, Human (Cat. No. UA011102) with EC50 of 5.75-7.71ng/mL.

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