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物种
Mouse分子别名
Bp50, CDW40, MGC9013, TNFRSF5, p50Accession
P27512-1表达序列
Val24-Arg193 with Human IgG1 Fc at C-Terminus
表达宿主
HEK293分子量
50-55kDa (Reducing)
纯度
＞95% by SDS-PAGE & SEC-HPLC活性
Immobilized CD40 Fc Chimera Protein, Mouse (Cat. No. UA011090) at 2.0μg/mL (100μL/well) can bind Mouse CD40 Ligand/TNFSF5 Protein with EC50 of 2.81-3.66ng/mL.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Hanlon MM, Smith CM, Canavan M, Neto NGB, Song Q, Lewis MJ, O'Rourke AM, Tynan O, Barker BE, Gallagher P, Mullan R, Hurson C, Moran B, Monaghan MG, Pitzalis C, Fletcher JM, Nagpal S, Veale DJ, Fearon U. Loss of synovial tissue macrophage homeostasis precedes rheumatoid arthritis clinical onset. Sci Adv. 2024 Sep 27;10(39):eadj1252.
CD40, also known as TNFRSF5, Bp50, CDW40, MGC9013, or p50, is a member of the tumor necrosis factor (TNF) receptor superfamily. This family consists of single transmembrane-spanning glycoproteins that play crucial roles in mediating a broad range of immune and inflammatory responses. CD40 is particularly important for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
CD40 features four cysteine-rich repeats in its extracellular domain and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain of CD40 contains cysteine-rich repeat regions, which are characteristic of many receptors within the TNF superfamily.
The interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules. These aggregated molecules then interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system highlighted its role in humoral immunity. Defects in the CD40 pathway can result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder. This condition is characterized by an inability of B cells to undergo isotype switching and to mount an antibody-specific immune response, as well as a lack of germinal center formation.
电泳JSON
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱JSON(SEC-HPLC)
The purity of CD40 Fc Chimera Protein, Mouse is more than 95% determined by SEC-HPLC.
ELISA
Immobilized CD40 Fc Chimera Protein, Mouse (Cat. No. UA011090) at 2.0μg/mL (100μL/well) can bind Mouse CD40 Ligand/TNFSF5 Protein with EC50 of 2.81-3.66ng/mL.
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