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物种
Mouse分子别名
rHuB7-2; Activation B7-2 antigen; B70; BU63; CD28LG2Accession
Q549Q9表达序列
Val24-Glu245, with C-terminal Fc表达宿主
HEK293分子量
65-75kDa (Reducing)纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
Immobilized B7-2/CD86 Fc Chimera Protein, Mouse (Cat. No. UA011076) at 2.0μg/mL (100μL/well) can bind Biotinylated CTLA-4/CD152 Fc Chimera, Mouse (Cat. No. UA011078) with EC50 of 30.32-62.10ng/mL.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Chia-Lin Chen, Jeffrey Y. Huang, Chun-HsiangWang, Stanley M Tahara, Lin Zhou, Yasuteru Kondo, Joel Schechter, Lishan Su,Michael M C. Lai, Takaji Wakita, François-Loïc Cosset, Jae U Jung & KeigoMachida: Hepatitis C virus has a genetically determined lymphotropism throughco-receptor B7.2, NatureCommunications volume 8, Article number: 13882 (2017).
CD86, also known as B-lymphocyte activation antigen B7-2 (formerly referred to as B70), is a member of the cell surface immunoglobulin superfamily. Predominantly existing as a monomer on cell surfaces, B7-2 interacts with two co-stimulatory receptors: CD28 and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), which are expressed on T cells. This interaction initiates signal pathways that regulate T cell activation and tolerance, cytokine production, and the generation of cytotoxic T lymphocytes (CTLs). B7 family members are transmembrane cell surface molecules that play crucial roles in immune activation and the maintenance of immune tolerance.
B7-2 is highly expressed on activated antigen-presenting cells (APCs), such as B cells, dendritic cells, and monocytes, as well as on vascular endothelial cells. B7-2 and its closely related counterpart, B7-1/CD80, exhibit overlapping yet distinct functional characteristics.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized B7-2/CD86 Fc Chimera Protein, Mouse (Cat. No. UA011076) at 2.0μg/mL (100μL/well) can bind Biotinylated CTLA-4/CD152 Fc Chimera, Mouse with EC50 of 30.32-62.10ng/mL.
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