IL-13RA1 His Tag Protein, Cynomolgus
L-13 receptor subunit alpha-1,IL-13R subunit alpha-1,IL-13R-alpha-1,IL-13RA1,Cancer/testis antigen 19,CD213a1,Interleukin 13 Receptor Subunit Alpha 1,CD213a1 Antigen,Interleukin-13 Receptor Subunit Alpha-1,Interleukin 13 Receptor,Alpha 1,IL-13 Receptor Subunit Alpha-1,IL13 Receptor Alpha-1 Chain,Cancer/Testis Antigen 19,IL-13R Subunit Alpha-1,IL-13Ra,NR4,IL-13R-Alpha-1,CD213A1,IL13RA
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物种
Cynomolgus分子别名
L-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Cancer/testis antigen 19, CD213a1, Interleukin 13 Receptor Subunit Alpha 1, CD213a1 Antigen, Interleukin-13 Receptor Subunit Alpha-1, Interleukin 13 Receptor, Alpha 1, IL-13 Receptor Subunit Alpha-1, IL13 Receptor Alpha-1 Chain, Cancer/Testis Antigen 19, IL-13R Subunit Alpha-1, IL-13Ra, NR4, IL-13R-Alpha-1, CD213A1, IL13RAAccession
XP_005594500.1表达序列
Ala25-Thr341 with His Tag at the C-Terminus表达宿主
HEK293分子量
55-72kDa (Reducing)
纯度
>95% by SDS-PAGE & SEC-HPLC内毒素含量
<0.1EU/μg活性
Protein A Chip captured IL-13 Protein, Human (Cat. No. UA040224), can bind IL-13RA1 His Tag Protein, Cynomolgus (Cat. No. UA011075) with an affinity constant of 0.52μM as determined in SPR assay.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Shi J., Song X., Traub B., Luxenhofer M., Kornmann M. Involvement of IL-4, IL-13 and Their Receptors in Pancreatic Cancer. Int. J. Mol. Sci. 2021;22:2998.
Interleukin 13 Receptor, Alpha 1 (IL13RA1), also referred to as NR4 or CD213A1 (Cluster of Differentiation 213A1), is a subunit of the Interleukin 13 Receptor complex. The IL13RA1 gene encodes a precursor protein consisting of 427 amino acid (aa) residues. This includes a 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region, and a 59 aa residue cytoplasmic tail.
IL13RA1 plays a crucial role in immune response by forming a receptor complex with the IL4 receptor alpha, a subunit common to both IL13 and IL4 receptors. As the primary IL13-binding subunit, IL13RA1 is integral to the IL13 receptor's function and may also contribute to the structure of IL4 receptors. The IL13RA1 protein has been demonstrated to bind with the tyrosine kinase TYK2, potentially mediating signaling pathways that activate JAK1, STAT3, and STAT6, which are induced by both IL13 and IL4.
Furthermore, IL13RA1 exhibits low-affinity binding to Interleukin-13 (IL13) and, in conjunction with the IL4 receptor alpha (IL4RA), forms a functional receptor for IL13. It also serves as an alternative accessory protein to the common cytokine receptor gamma chain (γc) in Interleukin-4 (IL4) signaling. However, it is important to note that IL13RA1 cannot replace the function of the Interleukin-2 Receptor Gamma (IL2RG) in enhancing Interleukin-2 (IL2) binding activity.
In the context of pancreatic cancer, IL-4 and IL-13 primarily exert their effects through their receptor heterodimers, IL-4 receptor alpha (IL-4Rα) and IL-13Rα1, collectively termed type II IL-4R. They activate signal transduction pathways including STAT3/6, IRS-ERK/PI3K-Akt, and mTOR, which are critical in the cellular response to these cytokines.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of IL-13RA1 His Tag Protein, Cynomolgus is more than 95% determined by SEC-HPLC.
SPR
Protein A Chip captured IL-13 Protein, Human (Cat. No. UA040224), can bind IL-13RA1 His Tag Protein, Cynomolgus (Cat. No. UA011075) with an affinity constant of 0.52μM as determined in SPR assay.
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