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物种
Mouse分子别名
Prostate stem cell antigen，UNQ206/PRO232，PRO232Accession
P57096表达序列
Leu21-Asn95, with C-terminal His表达宿主
HEK293分子量
20-30kDa (Reducing)
纯度
＞95% by SDS-PAGE &＞90% by SEC-HPLC内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, 5% trehalose, PH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Reiter RE, Gu Z, Watabe T, Thomas G, Szigeti K, Davis E, Wahl M, Nisitani S, Yamashiro J, Le Beau MM, Loda M, Witte ON. Prostate stem cell antigen: a cell surface marker overexpressed in prostate cancer. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1735-40.
2.Yang D, Holt GE, Velders MP, Kwon ED, Kast WM. Murine six-transmembrane epithelial antigen of the prostate, prostate stem cell antigen, and prostate-specific membrane antigen: prostate-specific cell-surface antigens highly expressed in prostate cancer of transgenic adenocarcinoma mouse prostate mice. Cancer Res. 2001 Aug 1;61(15):5857-60.
Prostate stem cell antigen (PSCA), a cell-membrane glycoprotein composed of 123 amino acids, is frequently expressed in prostate cancer. Mouse PSCA (mPSCA) exhibits 65% homology with human PSCA at both the nucleotide and amino acid levels. The knockdown of PSCA has been shown to induce epithelial-mesenchymal transition (EMT) and to reduce the metastatic potential of DU145 cells, indicating a significant role for PSCA in the carcinogenesis and progression of prostate cancer.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of PSCA His Tag Protein, Mouse is more than 90% determined by SEC-HPLC.
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