GSPT1/ERF3A GST&His Protein, Human
ERF3A,eRF3aFLJ38048,ETF3A,eukaryotic peptide chain release factor GTP-binding subunit ERF3A,Eukaryotic peptide chain release factor subunit 3a,FLJ39067,G1 to S phase transition 1,551G9.2,G1 to S phase transition protein 1 homolog,GST1
分享产品介绍 评论(0)
物种
Human分子别名
ERF3A,eRF3aFLJ38048,ETF3A,eukaryotic peptide chain release factor GTP-binding subunit ERF3A,Eukaryotic peptide chain release factor subunit 3a,FLJ39067,G1 to S phase transition 1,551G9.2,G1 to S phase transition protein 1 homolog,GST1Accession
P15170-1表达序列
Phe294-Asp499, with C-terminal GST&His表达宿主
Baculovirus-InsectCells分子量
51kDa (Reducing)纯度
＞85% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated性状
Liquid缓冲体系
50mM Tris, 20mM GSH, 5%Glycerol, pH8.0储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Cell. 2016 Nov 17;167(5):1229-1240.e15.
Cell. 2006 Jun 16;125(6):1125-36.
GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site.GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Binding of eRF1, eRF3, and GTP to pretermination complexes first induces a structural rearrangement that is manifested as a 2 nucleotide forward shift of the toeprint attributed to pretermination complexes that leads to GTP hydrolysis followed by rapid hydrolysis of peptidyl tRNA.
电泳
1μg (R: reducing condition, N: non-reducing condition).
评论(0)