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物种
Mouse分子别名
CD30; CD30KI-1; CD30L receptor; D1S166EKi-1; TNFRSF8Accession
Q60846表达序列
Phe19-Thr281, with C-hIgG Fc表达宿主
HEK293分子量
72-95kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg性状
Lyophilized Powder缓冲体系
PBS, 5% trehalose, PH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174
2. Kennedy, M.K. et al. (2006) Immunology 118:143.
3.Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily. Mature mouse CD30 consists of a 264 amino acid (aa) extracellular domain (ECD) with three cysteine-rich repeats, a 27 aa transmembrane segment, and a 190 aa cytoplasmic domain. In contrast, human CD30 includes an additional 90 aa in the ECD and contains six cysteine-rich repeats. CD30 is normally expressed on antigen-stimulated Th cells and B cells. However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity. CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells. In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3,and CCL5. An 85 ‑ 90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage. Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling.
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of CD30/TNFRSF8 Fc Chimera, Mouse is more than 90% determined by SEC-HPLC.
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