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产品中心 药物靶点和Fc受体 药物靶点

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NPC1 His Tag Protein, Human

NPC1,NPC Protein

价格 ￥1,760.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010945
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    NPC1, NPC Protein

  • Accession

    O15118-1

  • 表达序列

    Arg372-Phe622, with N-terminal His

  • 表达宿主

    HEK293

  • 分子量

    40-50 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & >95% by SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4,5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    J P Davies, Y A Ioannou. Dupilumab for the treatment of asthma. Topological analysis of Niemann-Pick C1 protein reveals that the membrane orientation of the putative sterol-sensing domain is identical to those of 3-hydroxy-3-methylglutaryl-CoA reductase and sterol regulatory element binding protein cleavage-activating protein.J Biol Chem. 2000 Aug 11;275(32):24367-74.

背景介绍

  • The Niemann-Pick C1 (NPC1) protein is predicted to be a polytopic glycoprotein, and it contains a region with extensive homology to the sterol-sensing domains (SSD) of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-R) and sterol regulatory element binding protein cleavage-activating protein (SCAP). To aid the functional characterization of NPC1, a model of NPC1 topology was evaluated by expression of epitope-tagged NPC1 proteins and investigation of epitope accessibility in selectively permeabilized cells. These results were further confirmed by expression of NPC1 and identification of glycosylated domains that are located in the lumen of the endoplasmic reticulum. Our data indicate that this glycoprotein contains 13 transmembrane domains, 3 large and 4 small luminal loops, 6 small cytoplasmic loops, and a cytoplasmic tail. Furthermore, our data show that the putative SSD of NPC1 is oriented in the same manner as those of HMG-R and SCAP, providing strong evidence that this domain is functionally important.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of NPC1 His Tag, Human is greater than 95% as determined by SEC-HPLC.

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