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物种
Mouse分子别名
CD300g, Nepmucin, CLM9Accession
NP_001154183.1表达序列
Leu19-Ile284 with Human IgG1 Fc at C-Terminus表达宿主
HEK293分子量
60-95kDa (Reducing)纯度
＞95% by SDS-PAGE & ＞90% by RP-HPLC标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Soojung Jin. Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro. FEBS Lett. 2008 Sep 3;582(20):3018-24. Epub 2008 Aug 4.
Nepmucin/CLM-9 is an Ig domain-containing sialomucin that is expressed in vascular endothelial cells. Our studies demonstrate that, similar to CD31, nepmucin is localized at interendothelial junctions and vesicle-like structures along the cell border, where it undergoes intracellular recycling. Functional analyses reveal that nepmucin mediates both homotypic and heterotypic cell adhesion through its Ig domain. Endothelial cells expressing nepmucin exhibit enhanced lymphocyte transendothelial migration (TEM), which can be inhibited by anti-nepmucin monoclonal antibodies (mAbs) that block either homophilic or heterophilic binding. Notably, mAbs that inhibit homophilic binding are able to block TEM without affecting lymphocyte adhesion. These findings suggest that endothelial nepmucin facilitates lymphocyte TEM through multiple adhesion pathways.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
The purity of CD300g/Nepmucin/CLM9 Fc Chimera Protein, Mouse is more than 90% as determined by RP-HPLC.
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