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物种
Human分子别名
CD300A,CLM-8,CMRF-35-H9,CMRF35-H9,CMRF35-H,IRC1/IRC2,IgSF12,IRp60,CMRF35H,IGSF12,LMIR1Accession
Q9UGN4-1表达序列
Leu18-Gln178, with C-terminal His表达宿主
HEK293分子量
25-43kDa (Reducing)纯度
＞95% by SDS-PAGE&＞95% by SEC-HPLC内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized powder缓冲体系
PBS, 5% trehalose, PH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Clark, G.J. et al. (2009) Trends Immunol. 30:209. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
LMIR1, also known as CD300a, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily. In human, LMIR1 is expressed on peripheral blood eosinophils, mast cells, neutrophils, plasmacytoid dendritic cells, and various T cell subsets.Antibody cross‑linking of LMIR1 induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation. Cross‑linking of LMIR1 to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors. LMIR1 cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms.CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).
电泳
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of is more than 95% as determined by SEC-HPLC.
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