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物种
Human分子别名
HGFR, c-MET, HGF/SF receptor, DFNB97, AUTS9, RCCP2, Hepatocyte growth factor receptor, Tyrosine-protein kinase MetAccession
P08581表达序列
Glu25-Thr932 with Human IgG1 Fc at C-Terminus表达宿主
HEK293分子量
34-48 kDa and 105-125 kDa (Reducing)
纯度
> 95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
Immobilized HGF Protein, Human (Cat. No. UA040194) at 2.0μg/mL (100μL/well) can bind HGFR/c-MET Fc Chimera Protein, Human (Cat. No. UA010829) with EC50 of 23.39-29.12ng/mL.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Neculai D. et al. (2013) Structure of LIMP-2 provides functional insights with implications for SR-BI and CD36. Nature. 504: 172-176.
McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
Garcia S. et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-335.
Socoteanu M P. et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-2994.
Kong D S. et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
HGF R, also known as Met, is a glycosylated receptor tyrosine kinase that plays a pivotal role in epithelial morphogenesis and cancer development. It is synthesized as a single-chain precursor, which undergoes cotranslational proteolytic cleavage to form a mature HGFR. This mature receptor is a disulfide-linked dimer consisting of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain. The extracellular domain (ECD) features a seven-bladed beta-propeller sema domain, a cysteine-rich PSI/MRS domain, and four Ig-like E-set domains. The cytoplasmic region contains the tyrosine kinase domain. HGFR is typically expressed by cells of epithelial origin, while HGF expression is limited to cells of mesenchymal origin.
电泳
1μg (R: reducing condition).
ELISA
Immobilized HGF Protein, Human (Cat. No. UA040194) at 2.0μg/mL (100μL/well) can bind HGFR/c-MET Fc Chimera Protein, Human (Cat. No. UA010829) with EC50 of 23.39-29.12ng/mL.
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