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物种
Cynomolgus分子别名
NKG2D,CD314,KLRK1,NK cell receptor DAccession
P61252表达序列
Phe78-Val216, with N-MD and hIgG1 Fc表达宿主
HEK293分子量
55-70kDa (Reducing)纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4.溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. 2.Diefenbach, A. et al. (2001) Nature 413:165. 3.NKG2D and its Ligands (2002) www.rndsystems.com.
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human ligands for NKG2D include MICA, MICB, and ULBP1,2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
ELISA
Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Cynomolgus/Rhesus (Cat. No. UA010739) with EC50 of 0.14-0.18 μg/mL.
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