NKG2D/CD314 Fc Chimera Protein, Human
Killer cell lectin-like receptor subfamily K member 1,NK cell receptor D,NKG2-D-activating NK receptor,CD314,D12S2489E,KLR,DNA Segment On Chromosome 12 (Unique) 2489 Expressed Sequence,Killer Cell Lectin Like Receptor K1,NKG2-D,Killer Cell Lectin-Like Receptor Subfamily K,Member 1,KLRK1,CD314 Antigen
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物种
Human分子别名
Killer cell lectin-like receptor subfamily K member 1，NK cell receptor D，NKG2-D-activating NK receptor，CD314，D12S2489E，KLR，DNA Segment On Chromosome 12 (Unique) 2489 Expressed Sequence，Killer Cell Lectin Like Receptor K1，NKG2-D，Killer Cell Lectin-Like Receptor Subfamily K， Member 1，KLRK1，CD314 AntigenAccession
P26718-1表达序列
Phe78-Val216, with N-MD and hIgG1 Fc表达宿主
HEK293分子量
50-70kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521. 2.Diefenbach, A. et al. (2001) Nature 413:165. 3.NKG2D and its Ligands (2002) www.rndsystems.com
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.
流式分析
2e5 of transient transfected anti-CD314 ScFv CAR-293 cells were stained with 0.01ug NKG2D/CD314 Fc Chimera Protein, Human, (Cat. No. UA010732) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- CD314 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized MICB His Tag, Human (Cat. No. UA010590) at 2.0μg/mL (100μL/well) can bind NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732) with EC50 of 0.48-0.61μg/mL.
Immobilized NKG2D/CD314 Fc Chimera Protein, Human (Cat. No. UA010732) at 2.0μg/mL (100μL/well) can bind MICA His Tag Protein, Human (Cat. No. UA011069) with EC50 of 0.093-0.15μg/mL .
SPR
Protein A Chip captured NKG2D/CD314 Fc Chimera, Human (Cat. No. UA010732), can bind MICB His Tag, Human (Cat. No. UA010590) with an affinity constant of 47.67nM as determined in SPR assay.
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