Biotinylated CD52 Fc&Avi Tag Protein, Human
Epididymis Secretory Sperm Binding Protein Li 171mP,CD52 Antigen (CAMPATH-1 Antigen),Epididymal Secretory Protein E5,He5,CDW52 Antigen,HEL-S-171mP,EDDM5,CDw52,Cambridge pathology 1 antigen,Human epididymis-specific protein 5,CD52 Molecule
分享产品介绍 评论(0)
物种
Human分子别名
Epididymis Secretory Sperm Binding Protein Li 171mP,CD52 Antigen (CAMPATH-1 Antigen),Epididymal Secretory Protein E5,He5,CDW52 Antigen,HEL-S-171mP,EDDM5,CDw52,Cambridge pathology 1 antigen,Human epididymis-specific protein 5,CD52 MoleculeAccession
P31358表达序列
Gly25-Ser36, with C-terminal human IgG1 Fc &Avi tag表达宿主
HEK293分子量
35-43kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Biotin标签
Mouse Fc Tag, Avi Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Koyama K. et al. (2009) Functional aspects of CD52 in reproduction. J Reprod Immunol. 83(1-2): 56-59. 2. Morris, P.J. and N.K. Russell (2006) Transplantation 81:1361. 3. Redpath, S. et al. (1998) Immunology 93:595. 4. Hardiyanto, L. et al. (2012) J. Reprod. Immunol. 94:142.
CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis. CD52 is a GPI-anchored protein present in lymphocytes and male reproductive tissues (mrt) including mature sperm and seminal plasma. It has been shown that mrt-CD52 is synthesized in epithelial cells of the epididymis and vas deferens, but not in the testis. The mrt-CD52 is transported to mature sperm during sperm transition in the male reproductive tract. Lymphocyte CD52 functions to stimulate suppressor T cell induction, while mrt-CD52 is associated with seminogelin and involved in clot formation and liquefaction of semen. It protects sperm against anti-sperm antibodies by binding to C1q and inhibiting complement activation.
电泳
1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized Biotinylated CD52 Fc&Avi Tag Protein, Human (Cat. No. UA010691) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind CD52 Recombinant Mouse mAb (S-R431) (Cat. No. S0B0685) with EC50 of 6.88-13.26ng/ml.
评论(0)