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物种
Cynomolgus分子别名
CD210 antigen, CDw210a, HIL-10R, IBD28, IL-10 R alpha, IL-10 receptor subunit alpha, IL10R alpha, IL-10R subunit 1, IL-10R1, IL10RA, IL-10Ra, IL10RIL-10R subunit alphaAccession
XP_005579838.2表达序列
His22-Asn235, with C-terminal Human IgG1 Fc HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGTGRWNSISNCSQALSYDLTAVTLDLYRSNGYRARVRAVDGSRHSNWTVTNTRFSLDEVTLTVGSVKLEIHNGFILGKIQPPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVNHENFSLLTSGEVGEFCVQVKPSVTSRTNKGMWSKEECVSLTRQYFTVTNIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
表达宿主
HEK293分子量
72-90kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
2.Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
3.Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
4.Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
5.Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 51 kDa transmembrane glycoprotein member of the class II cytokine receptor family. IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Interleukin-10 receptor subunit alpha (IL-10 R alpha) is a cell surface receptor for the cytokine IL-10 that participates in IL-10 mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3.
电泳
2μg (R: reducing condition, N: non-reducing condition).
SPR
Protein A Chip captured IL10RA/CD210 Fc Chimera, Cynomolgus (Cat. No. UA010643), can bind IL-10 Protein, Cynomolgus (Cat. No. UA040260) with an affinity constant of 0.56nM as determined in SPR assay.
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