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物种
Human抗原名称
CD48分子别名
BCM1, SLAMF2, BLAST, BLAST1, MEM-102, TCT.1, BCM-1, SLAMF-2Accession
P09326-1表达序列
Gln27-Ser220, with C-terminal 8*His Tag表达宿主
HEK293分子量
35-45kDa纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Shannon L McArdel. Roles of CD48 in regulating immunity and tolerance. Clin Immunol. 2016 Mar; 164:10-20.Epub 2016 Jan 18.
CD48, a member of the signaling lymphocyte activation molecule family, participates in adhesion and activation of immune cells. Although constitutively expressed on most hematopoietic cells, CD48 is upregulated on subsets of activated cells. CD48 can have activating roles on T cells, antigen presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD244:CD48 interactions regulate target cell lysis by NK cells and CTLs, which are important for viral clearance and regulation of effector/memory T cell generation and survival.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized CD48 His Tag, Human (Cat. No. UA010595) at 2.0μg/mL (100μL/well) can bind 2B4/CD244 Fc Chimera, Human(Cat. No. UA010890) with EC50 of 36.94-47.14 ng/mL.
SPR
Protein A Chip captured 2B4/CD244 Fc Chimera, Human (Cat. No. UA010890), can bind CD48 His Tag, Human(Cat. No. UA010595) with an affinity constant of 17.70nM as determined in SPR assay.
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