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物种
Human抗原名称
CD39/ENTPD1分子别名
CD39, Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1Accession
P49961-1表达序列
Thr38 -Val478, with C-terminal 10*His表达宿主
HEK293分子量
70-80kDa
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Grinthal, A. and G. Guidotti. (2004) Biochemistry 43:13849. 2.Robson, S.C. et al. (2006) Purinergic Signal. 2:409. 3.Zebisch, M. et al. (2012) J. Mol. Biol. 415:288.
CD39, also known as ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1), is the prototypical member of the NTPDase family. CD39 is a metal-dependent, homodimeric enzyme bound to the cell membrane through two transmembrane domains present at each termini. CD39 hydrolyzes the beta - and gamma phosphate residues of nucleotides to terminate purinergic signaling and is uniquely capable of processive hydrolysis of extracellular ATP to AMP without release of the ADP intermediate as the initial rate-limiting steps in the production of extracellular adenosine. CD39 ecto-nucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.
电泳
1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized CD39/ENTPD1 His Tag Protein, Human (Cat. No. UA010585) at 2.0μg/mL (100μL/well) can bind CD39 Recombinant Rabbit mAb (SDT-236-20) (Cat. No. S0B0050) with EC50 of 0.76-1.17ng/mL.
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