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物种
Human分子别名
EHK-1,EK7,BSK,EHK1,HEK7,TYRO4,Rek7,TYRO4HEK7CEK7Accession
P54756表达序列
Pro25-Pro573, with C-terminal 10*His表达宿主
HEK293分子量
72-85kDa纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. CHENGYING LIU, YAN HE, XIAO FENG, et al. Expression of EPHA5 in lung adenocarcinoma is associated with lymph node metastasis and EGFR mutation[J]. 2022,130(6):338-345.
EPHA5 is a member of the Eph family of tyrosine kinase receptors, which affect carcinogenesis. High expression of EPHA5 was found in NCI‐H460 and H1299 cells, while low expression was observed in A549 and SPC‐A1 cells. EPHA5 was knocked down in NCI‐H460 and H1299 lung cancer cell lines using siRNAs. EPHA5 receptor is differently expressed in lung adenocarcinoma tissues, in which positive and negative expression of EPHA5 was found in 58.1% and 41.9% of tissues, respectively.EPHA5 receptor plays a role in tumor promotion in lung adenocarcinoma and it is a potential target for lung adenocarcinoma treatment.
电泳
2μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized EphA5 His Tag, Human (Cat. No. UA010566) at 2.0μg/mL (100μL/well) can bind Ephrin-A3 Fc Chimera, Human (Cat. No. UA010141) with EC50 of 0.50-0.68μg/mL .
分子互作
Protein A Chip captured Ephrin-A3 Fc Chimera, Human (Cat. No. UA010141), can bind EphA5 His Tag, Human (Cat. No. UA010566) with an affinity constant of 19.49 nM as determined in SPR assay.
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