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物种
Mouse抗原名称
TNFRSF10B/TRAIL R2分子别名
TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5,KILLER,TRICK2,ZTNFR9,TRICKBAccession
Q9QZM4表达序列
Asn53-Ser177, with C-terminal 8*His表达宿主
HEK293分子量
20-33kDa纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Walczak, H. et al. (1997) EMBO J. 16:5386.
TNFRSF10B, also called TRAIL R2 and DR5, is a member of the TNF receptor superfamily. TRAIL-R2 contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. Some studies have suggested that TNFRSF10B contributes more than TNFRSF10A (TRAIL R1) to TRAIL-induced apoptosis in cancer cells that express both receptors. In addition, agonistic monoclonal antibodies against TNFRSF10B, which can induce apoptosis in the absence of TRAIL, are used for cancer therapies.
电泳
- 2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized TRAIL/Apo2L, Human (Cat. No. UA040039) at 2.0μg/mL (100μL/well) can bind TNFRSF10B/TRAIL R2 His Tag, Mouse (Cat. No. UA010509) with EC50 of 30.69-44.23 ng/ml.
SPR
Anti-His antibody Immobilized on CM5 Chip captured TNFRSF10B/TRAIL R2 His Tag, Mouse (Cat. No. UA010509), can bind TRAIL/Apo2L, Human (Cat. No. UA040039) with an affinity constant of 4.67nM as determined in SPR assay.
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