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物种
Human抗原名称
TSLPR分子别名
CRLF2,CRL2,ILXR,TSLPRAccession
Q9HC73表达序列
Gln23-Lys231, with C-terminal 8* His表达宿主
HEK293分子量
33-43kDa (Reducing)纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized powder缓冲体系
PBS, pH7.4溶解方法
Reconstituteat 0.1-1 mg/ml according to the size in ultrapure water after rapidcentrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.L S Park. Cloning of the murine thymic stromallymphopoietin (TSLP) receptor: Formation of a functional heteromeric complexrequires interleukin 7 receptor. J Exp Med. 2000 Sep 4;192(5):659-70.
The TSLP receptor (TSLPR, CRLM-2), a typical heterodimeric cytokine receptor consisting of a TSLP binding subunit (TSLPRα) and the α-subunit of the IL-7 receptor (IL-7Rα). TSLPR mRNA has been detected on many immune cell types, including dendritic cells (DCs), T cells, B cells, mast cells, NKT cells and monocytes as well as in tissues from heart, skeletal muscle, kidney and liver. TSLPR has low affinity for TSLP, but in combination with IL-7Rα generates a high affinity binding site for TSLP and triggers signaling. The TSLPR regulates target gene expression via the JAK/STAT signalling pathway with participation of signal transducers and activators of transcription STAT3, STAT5 and STAT1. Genetic, experimental, and clinical evidence suggests that the TSLP-TSLPR pathway is associated with the pathogenesis of allergic diseases such as atopic dermatitis (AD) and asthma.
生物活性
Measured by its binding ability in a functional ELISA. When TSLP, Human(Cat.No:UA040102) is immobilized 10µg/ml (100µl/well), TSLPR His Tag, Human binds with an EC50 of 1.5-2.0μg/ml.
Anti-His antibody Immobilized on CM5 Chip captured TSLPR His Tag, Human (Cat. No. UA010490), can bind TSLP, Human (Cat. No. UA040102) with an affinity constant of 0.70 nM as determined in SPR assay.
电泳
1μg (R: reducing conditions, N: non-reducing conditions).
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