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产品中心 药物靶点和Fc受体 药物靶点

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Biotinylated Her2 His&Avi Tag Protein, Human

CD340,ERBB2,NEU,NGL,MLN19,TKR1

价格 ￥3,060.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010485
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 抗原名称

    Her2

  • 分子别名

    CD340, ERBB2, NEU, NGL, MLN19, TKR1

  • Accession

    P04626-1

  • 表达序列

    Thr23-Thr652, with C-terminal 8*His & Avi tag

    TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTGGGSHHHHHHHHGLNDIFEAQKIEWHE


  • 表达宿主

    HEK293

  • 分子量

    80-95kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Biotin

  • 标签

    Avi Tag, His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Antonio C. Wolff; M. Elizabeth H. Hammond; JaredN. Schwartz; Karen L. Hagerty; D. Craig Allred; Richard J. Cote; MitchellDowsett; Patrick L. Fitzgibbons; Wedad M. Hanna; Amy Langer; Lisa M. McShane;Soonmyung Paik; Mark D. Pegram; Edith A. Perez; Michael F. Press; AnthonyRhodes; Catharine Sturgeon; Sheila E. Taube; Raymond Tubbs; Gail H. Vance; Marcvan de Vijver; Thomas M. Wheeler; Daniel F. Hayes: American Society of ClinicalOncology/College of American Pathologists Guideline Recommendations for HumanEpidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med (2007) 131 (1): 18–43.

背景介绍

  • Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2, HER-2 /neu, NEU, NGL, TKR1 and c-erb B2, and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin.

  • 电泳

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized Biotinylated Her2 His&Avi Tag Protein, Human (Cat. No. UA010485) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Herceptin HER2 Trastuzumab (Cat. No. UA011035) with EC50 of 0.55-0.69ng/ml.

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