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物种
Human, Human抗原名称
MOG分子别名
MOG,BTN6,BTNL11,MOGIG2,NRCLP7,Myelin oligodendrocyte glycoproteinAccession
Q16653表达序列
Gly30-Gly154, with C-terminal 8*His
表达宿主
HEK293分子量
20-23kDa (Reducing)
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
Female C57BL/6 mice (8-10 weeks old) were immunized (s.c.) with 100 μg/animal of human rMOG in complete Freund’s adjuvant followed by 400 ng/mouse of pertussis toxin on day 0 and day 2 (i.p.). Mice showed EAE symptoms such as limp tail, hind limb weakness, or hind limb paralysis after induction. DA rats immunized with 50 μg/animal of human rMOG at the base of the tail (s.c.) displayed EAE symptoms as well.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to immunoglobulin superfamily. Mouse MOG is synthesized with a 28 amino acid (aa) signal sequence, a 128 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 69 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane. This makes MOG a potential target of cellular and humoral immune responses in inflammatory demyelinating diseases.
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys . The purified human rMOG is recommended for in vitro studies such as T cell and B cell responses, cytokine response, antigen presentation, Western blotting, and ELISA as well as for in vivo study such as EAE induction in mice and monkeys. The following dosages are recommended: 5-20 μg/ml for in vitro study and 50-100 μg per animal for in vivo study
生物活性
Data are from three independent groups in a single experiment. Immunization used MOG1-125/CFA Emulsion PTX , with 11 to 13 week old female C57BL/6 mice
电泳
1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized MOG(1-125) Protein, Human (Cat. No. UA010471) at 2.0μg/mL (100μL/well) can bind Myelin Oligodendrocyte Glycoprotein (MOG) Recombinant Rabbit mAb (S-1384-61) (Cat. No. S0B0812) with EC50 of 0.81-0.97ng/mL.
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