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物种
Human抗原名称
MOG分子别名
MOG,BTN6,BTNL11,MOGIG2,NRCLP7,Myelin oligodendrocyte glycoproteinAccession
Q16653表达序列
Gly30-Gly154, with C-terminal 8*His
表达宿主
HEK293分子量
20-23kDa (Reducing)
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to immunoglobulin superfamily. Mouse MOG is synthesized with a 28 amino acid (aa) signal sequence, a 128 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 69 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane. This makes MOG a potential target of cellular and humoral immune responses in inflammatory demyelinating diseases.
- Female C57BL/6 mice (8-10 weeks old) were immunized with 100 ug/animal of human rMOG in complete Freund's adjuvant followed by 400 ng/mouse of pertussis toxin on day 0 and day 2. Mice showed EAE symptoms such as limp tail, hind limb weakness, or hind limb paralysis after induction. Please note that no other EAE induction protocols were tested including IFA/cytokine model.
电泳(SDS-PAGE)
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1μg (R: reducing conditions, N: non-reducing conditions).
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