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物种
Human抗原名称
MSLN分子别名
CAK1 antigen,Pre-pro-megakaryocyte-potentiating factorAccession
Q13421-1表达序列
Glu296-Gly580, with C-terminal Human IgG1 Fc &Avi tag表达宿主
HEK293分子量
72-85kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Biotin标签
Human Fc Tag, Avi Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Hassan, R. et al: Mesothelin: a new target for immunotherapy, Clin.Cancer Res. 10:3937(2004).
Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized Anti-Human MSLN Monoclonal Antibody (Amatuximab) at 2.0μg/mL (100μL/well) can bind Biotinylated MSLN Fc&Avi Tag, Human (Cat. No. UA010459)with EC50 of 4.86-5.99ng/mL.
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