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物种
Mouse分子别名
GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYSAccession
Q8CFZ4表达序列
Gln25-Met557, with C-terminal 10*His
表达宿主
HEK293分子量
72-130kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Kandil D H. et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-129.
2、Maeda D. et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-832.
- Glypican-3 (GPC3) belongs to a six-member family of membrane-bound heparan sulfate proteoglycans with a gene that is localized to Xq26.2. GPC3 belongs to a family of Glypicans are heparan sulfate proteoglycans that are bound to the cell surface by glycosyl phosphatidyli nositol. While six members of the glypican family are known in mammals, our study focused on glypican 3(GPC3). Loss-of-function mutations of GPC3 result in the Simpson- Golabi-Behmel syndrome, an X-linked disorder characterized by pre- and postnatal liver and other organ overgrowth. GPC3 is overexpressed in human hepatocellular carcinoma; however, its role in normal liver regeneration and hepatocyte proliferation is unknown. GPC3 is located on the X chromosome, and is highly expressed during embryogenesis and organogenesis. In the adult, on the other hand, GPC3 can only be detected in a limited number of tissues, including the lung, ovaries, mammary epithelium and mesothelium.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized FGF-basic(146aa) Protein, Human (Cat. No. UA040145) at 2.0μg/mL (100μL/well) can bind GPC3/Glypican 3 His Tag Protein, Mouse (Cat. No. UA010371) with EC50 of 15.15-21.86 ng/mL.
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