CD52 Fc Chimera Protein, Human
Epididymis Secretory Sperm Binding Protein Li 171mP,CD52 Antigen (CAMPATH-1 Antigen),Epididymal Secretory Protein E5,He5,CDW52 Antigen,HEL-S-171mP,EDDM5,CDw52,Cambridge pathology 1 antigen,Human epididymis-specific protein 5,CD52
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物种
Human分子别名
Epididymis Secretory Sperm Binding Protein Li 171mP,CD52 Antigen (CAMPATH-1 Antigen),Epididymal Secretory Protein E5,He5,CDW52 Antigen,HEL-S-171mP,EDDM5,CDw52,Cambridge pathology 1 antigen,Human epididymis-specific protein 5,CD52Accession
P31358表达序列
Gly25-Ser36, with C-terminal Human IgG1 Fc GQNDTSQTSSPSIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
表达宿主
HEK293分子量
35-40kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Koyama K. et al. (2009) Functional aspects of CD52 in reproduction. J Reprod Immunol. 83(1-2): 56-59.
CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis. CD52 is a GPI-anchored protein present in lymphocytes and male reproductive tissues (mrt) including mature sperm and seminal plasma. It has been shown that mrt-CD52 is synthesized in epithelial cells of the epididymis and vas deferens, but not in the testis. The mrt-CD52 is transported to mature sperm during sperm transition in the male reproductive tract. Lymphocyte CD52 functions to stimulate suppressor T cell induction, while mrt-CD52 is associated with seminogelin and involved in clot formation and liquefaction of semen. It protects sperm against anti-sperm antibodies by binding to C1q and inhibiting complement activation.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized CD52 Fc Chimera Protein, Human (Cat. No. UA010305) at 2μg/mL (100μL/well) can bind CD52 Recombinant Mouse mAb (S-R431) (Cat. No. S0B0685) with EC50 of 19.86-24.09ng/ml.
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