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物种
Human分子别名
FcRn,FCGRT & B2MAccession
P55899-1表达序列
FCGRT Chain: Ala24-Ser297, with C-terminal 6*10 His
B2M Chain: Ile21-Met119
表达宿主
HEK293分子量
33-35 & 11-13kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
The neonatal Fc receptor (FcRn) is a mostly intracellularly expressed membrane- associated receptor, which is best known for mediating the extraordinarily long half-life of IgG and placental transport of thereof. FcRn complex consists of two subunits: p51, and p14 which is equivalent to beta-2-microglobulin, and forms an MHC class I-like heterodimer. The FcRn, is expressed in human placental syncytiotrophoblast, capillary endothelium, intestinal epithelium, and other tissues. FcRn dependent placental transport of IgE from mother to offspring in mice. The FcRn interacts with IgG and albumin at acidic pH within endosomes, thus protecting these plasma proteins from degradation to keep a long plasma half-life. FcRn binds IgG with nanomolar affinities at low pH (≤6.5) (found in intracellular vacuoles), while at physiological pH (7.4) this affinity is low.
生物活性
- Anti-His antibody Immobilized on CM5 Chip captured FcRn His Tag, Human (Cat. No. UA010279), can bind Anti-Human HER2(Trastuzumab) Monoclonal Antibody with an affinity constant of 0.3μM as determined in SPR assay.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
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