分享产品介绍 评论(0)
物种
Mouse抗原名称
PD-L1分子别名
PD-L1,B7-H1,CD274,PDCD1L1,PDCD1LG1Accession
Q9EP73表达序列
Phe19-Thr238, with C-terminal Human IgG1 Fc表达宿主
HEK293分子量
64-78kDa纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Salih H R. et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-894.
2、Wilcox R A. et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-2158.
3、Ruggiero A. et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-1142.
Programmed death ligand 1 (PD-L1) belongs to the B7 series and is a 33-kDa type 1 transmembrane glycoprotein that contains 290 amino acids with Ig-V and IgC domains in its extracellular region. PD-L1 expression can be detected on hematopoietic cells including T cells, B cells, macrophages, dendritic cells (DCs), and mast cells, and non-hematopoietic healthy tissue cells including vascular endothelial cells, keratinocytes, pancreatic islet cells, astrocytes, placenta syncytiotrophoblast cells, and corneal epithelial and endothelial cells. PD-L1 is an essential immune checkpoint protein that binds to programmed death 1 (PD-1) on T-lymphocytes. Engagement of PD-1 by PD-L1 alters the activity of T cells in many ways, inhibiting T cell proliferation, survival, cytokine production, and other effector functions. T cell plays a critical role in killing cancer cells while the cancer cell exhibits immune escape by the expression of PD-L1. The binding of PD-L1 to PD-1 inhibits T cell proliferation and activity, leading to tumor immunosuppression.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized PD-1 His Tag, Mouse (Cat. No. UA010292) at 2.0μg/mL (100μL/well) can bind PD-L1/B7-H1 Fc Chimera, Mouse (Cat. No. UA010259) with EC50 of 2.18-3.03μg/ml.
SPR
Protein A Chip captured PD-L1/B7-H1 Fc Chimera, Mouse (Cat. No. UA010259), can bind PD-1 His Tag, Mouse (Cat. No. UA010292) with an affinity constant of 0.42μM as determined in SPR assay.
评论(0)