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产品中心 药物靶点和Fc受体 药物靶点

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HGFR/c-MET His Tag Protein, Human

HGFR,c-MET,HGF/SF receptor,DFNB97,AUTS9,RCCP2,Hepatocyte growth factor receptor,Tyrosine-protein kinase Met

价格 ￥2,780.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010226
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    HGFR,c-MET,HGF/SF receptor,DFNB97,AUTS9,RCCP2,Hepatocyte growth factor receptor,Tyrosine-protein kinase Met

  • Accession

    P08581-1

  • 表达序列

    Glu25-Thr932, with C-10*His

  • 表达宿主

    HEK293

  • 分子量

    80-90kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Garcia S. et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-335.

    2、Socoteanu M P. et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-2994.

    3、Kong D S. et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.

    4、McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.

背景介绍

  • HGF R, also known as Met, is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGFR that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain.. The extracellular domain (ECD) contains a seven bladed beta-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin.

  • 电泳

    • 1μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized HGFR/c-MET His Tag Protein, Human (Cat. No. UA010226) at 2.0μg/mL (100μL/well) can bind Anti-Human MET (Emibetuzumab) with EC50 of 2.00-3.30ng/mL.

    • Immobilized HGFR/c-MET His Tag Protein, Human (Cat. No. UA010226) at 2.0μg/mL (100μL/well) can bind HGF Fc Chimera Protein, Human with EC50 of 0.27-0.71ng/mL.

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