热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 药物靶点和Fc受体 药物靶点

所有图片（1/2）

RNF43 mFc Chimera Protein, Mouse

RNF43,RING finger protein 43

价格 ￥2,800.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010216
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    RNF43,RING finger protein 43

  • Accession

    Q5NCP0

  • 表达序列

    Ala34-Tyr197, with C-terminal mIgG2a Fc

  • 表达宿主

    HEK293

  • 分子量

    55-60kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Mouse Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Radaszkiewicz T. et al. (2021) RNF43 inhibits WNT5A driven signaling and suppresses melanoma invasion. eLife Sciences. 10.

背景介绍
  • RNF43 is an E3 ubiquitin ligase and known negative regulator of WNT/β-catenin signaling. We demonstrate that RNF43 is also a regulator of noncanonical WNT5A- induced signaling in human cells. Analysis of the RNF43 interactome using BioID and immunoprecipitation showed that RNF43 can interact with the core receptor complex components dedicated to the noncanonical Wnt pathway such as ROR1, ROR2, VANGL1, and VANGL2. RNF43 triggers VANGL2 ubiquitination and proteasomal degradation and clathrin- dependent internalization of ROR1 receptor and inhibits ROR2 activation. These activities of RNF43 are physiologically relevant and block pro-metastatic WNT5A signaling in melanoma. RNF43 inhibits responses to WNT5A, which results in the suppression of invasive properties of melanoma cells.

  • 生物活性

    • RNF43 mFc Chimera, Mouse (Cat. No. UA010216) captured on Protein A Biosenor, can bind RSPO1(21-146) His Tag, Human (Cat. No. UA040022) with an affinity constant of 6.73nM as determined in SPR assay.

  • 电泳

    • 1μg (R: reducing conditions, N: non-reducing conditions).

评论(0)