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产品规格
物种
Human分子别名
TPK1,Thiamin pyrophosphokinase 1,hTPK1,Placental protein 20,PP20,Thiamine pyrophosphokinase 1Accession
Q9H3S4表达序列
Met1-Ser243, with C-8*His表达宿主
E.coli分子量
27-28kDa纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Mayr J A. et al. (2011) Thiamine pyrophosphokinase deficiency in encephalopathic children with defects in the pyruvate oxidation pathway. Am J Hum Genet. 89(6): 806-812.
2、Bellyei S.et al. (2005) Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK). Placenta. 26(1): 34-46.
背景介绍
- Thiamin pyrophosphokinase 1 (TPK1), a member of the thiamine pyrophosphokinase family, is also known as Thiamine pyrophosphokinase 1, Placental protein 20 (PP20) and hTPK1, which is detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues. TPK1 is regarded as a Kinase and a Transferase that can bind ATP and Nucleotide.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
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