分享产品介绍 评论(0)
物种
Human分子别名
CD28LG2,B7-2,CD86,B70,LAB72,MGC34413Accession
P42081表达序列
Leu26-Pro247, with C-hIgG1 Fc表达宿主
HEK293分子量
72-90kDa (Reducing)纯度
＞95% by SDS-PAGE
内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
CD86 is a well-known costimulatory molecule in its interaction with CD28 and/or CTLA present on T cells, and is essential for full activation of naive T-cell and subsequent differentiation. Usually, the B7 molecules are expressed mainly on APCs and B cells and in specific conditions on other activated cells. These costimulatory molecules are involved in the development of allergic inflammation and airways hyperreactivity (AHR) in allergen-challenged mice. Activated T cells, CD4+CD25+, express CD86 in the first 60 minutes after the specific inhalation exposure. These T cells can be relevant in IgE mediated allergic reaction possibly by an autocrine co-stimulation via CD28/CTLA activation pathway. The blockage of the expression of CD86 could be a potential therapeutical target to reduce the magnitude or the progression of the allergic reaction.
生物活性
- Anti-His antibody Immobilized on CM5 Chip captured CTLA-4 His Tag, Human (Cat. No. UA010077), can bind B7-2/CD86 Fc Chimera, Human (Cat. No. UA010187) with an affinity constant of 0.12μM as determined in SPR assay.
- Immobilized CTLA-4 His Tag, Human (Cat. No. UA010077) at 1.5μg/mL (100μL/well) can bind B7-2/CD86 Fc Chimera, Human (Cat. No. UA010187) with EC50 of 0.074-0.094μg/ mL
电泳
- 2μg (R: reducing conditions, N: non-reducing conditions).
评论(0)