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MSLN/Mesothelin (298-600) His Tag Protein, Mouse
规格：100μg
价格：￥3,000.00
MSLN/Mesothelin (296-580) His Tag Protein, Human
规格：100μg
价格：￥3,000.00
MSLN/Mesothelin (37-286) His Tag Protein, Human
规格：100μg
价格：￥3,000.00
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物种
Human分子别名
MSLN/Mesothelin,MPF,SMRPAccession
Q13421-1表达序列
Glu296-Gly580, with C-hIgG1 Fc表达宿主
HEK293分子量
70-85kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Chang K. et al. (1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. PNAS. 93(1): 136-140.
2、Liu X. et al. (2020) Multiple proteases are involved in mesothelin shedding by cancer cells. Commun Biol. 3: 728.
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte potentiating factor, which belongs to the mesothelin family. Mesothelin/MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin/MSLN can interacts with MUC16. The membrane-anchored form of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro. Mesothelin (MSLN) is a lineage restricted cell surface protein expressed in about 30% of human cancers and high MSLN expression is associated with poor survival in several different cancers. The restricted expression of MSLN in normal tissue and its frequent expression in cancers make MSLN an excellent target for antibody-based therapies.
流式分析
2e5 of transient transfected anti-MSLN ScFv CAR-293 cells were stained with 0.1ug MSLN/Mesothelin Fc Chimera Protein, Human, (Cat. No. UA010184) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity. 2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
生物活性
- Immobilized CA125/MUC16 His Tag, Human (Cat. No. UA010258) at 2.0μg/mL (100μL/well) can bind MSLN/Mesothelin Fc Chimera, Human (Cat. No. UA010184) with EC50 of 0.031-0.043μg/ml.
- Immobilized CA125/MUC16 His Tag, Cynomolgus (Cat. No. UA010192) at 1.5μg/mL (100μL/well) can bind MSLN/Mesothelin Fc Chimera, Human (Cat. No. UA010184) with EC50 of 0.52-0.72μg/ml.
Immobilized Biotinylated CA125/MUC16 His&Avi Tag, Human (Cat. No. UA010940) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind MSLN/Mesothelin Fc Chimera, Human (Cat. No. UA010184) with EC50 of 4.04-5.02 ng/ml.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
SPR
Anti-His antibody Immobilized on CM5 Chip captured CA125/MUC16 His Tag, Human (Cat. No. UA010258), can bind MSLN/Mesothelin Fc Chimera, Human (Cat. No. UA010184) with an affinity constant of 0.11μM as determined in SPR assay.
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