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产品规格
物种
Human分子别名
Antigen NY-CO-27,GAL4,gal-4,galectin 4,Galectin4,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4,lectin galactoside-binding soluble 4,LGALS4Accession
P56470表达序列
Ala2-Ile323表达宿主
E.coli分子量
36kDa纯度
>95% by SDS-PAGE
内毒素含量
<0.1EU/μg标记
Unconjugated性状
Lyophilized Powder缓冲体系
250mM Tris, 150mM NaCl, 3mM DTT, pH8.0溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Poland P A. et al. (2022) Cloning, Expression, and Purification of Galectins for In Vitro Studies. Methods in Molecular Biology. 2442: 41-54.
2、Rustiguel J K. et al. (2016) Recombinant expression, purification and preliminary biophysical and structural studies of C-terminal carbohydrate recognition domain from human galectin-4. 118: 39-48.
3、Stancic M. et al. (2012) Galectin-4, a Novel Neuronal Regulator of Myelination. GLIA. 60(6): 919-935.
背景介绍
- Galectins are best known for their ability to bind glycoconjugates containing β-galactose, but classification of these small proteins within the galectin family is also defined by amino acid homology within structural domains and exon/intron junctions within genes. Galectin-4 (Gal4), a tandem-repeat type galectin, is expressed in healthy epithelium of the gastrointestinal tract. Altered levels of Gal4 expression are associated with different types of cancer, suggesting its usage as a diagnostic marker as well as target for drug development. The functional data available for this class of proteins suggest that the wide spectrum of cellular activities reported for Gal4 relies on distinct glycan specificity and structural characteristics of its two carbohydrate recognition domains. Gal4, a tandem-repeat-type lectin with affinity to sulfatide and non-sialylated termini of N-glycans, has the ability to regulate adhesion of cells to ECM components and is also involved in polarized membrane trafficking.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
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