FAP His Tag Protein, Human

FAPA,SIMP,DPPIV,FAP alpha

浏览次数：31

货号： UA010165

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

物种

Human
分子别名

FAPA, SIMP, DPPIV, FAP alpha
Accession

Q12884
表达序列

Leu26-Asp760, with N-8*His
表达宿主

HEK293
分子量

80-90kDa
纯度

>95% by SDS-PAGE
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

His Tag
性状

Lyophilized powder
缓冲体系

PBS, pH7.4
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1、Cheng J D. et al. (2005) Fibroblast activation protein overexpression confers an enhanced adherence and motility phenotype. Cancer Res. 65(1): 1326.
2、Kraman M. et al. (2010) Suppression of Antitumor Immunity by Stromal Cells Expressing Fibroblast Activation Protein-α. Science. 330(6005): 827-830.
3、Bauer S. et al. (2006) Fibroblast activation protein is expressed by rheumatoid myofibroblast-like synoviocytes. Arthritis Res Ther. 8(6): R171.

背景介绍

Neuropeptide-associated fibroblast activation protein (FAP) is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Neuropeptide-associated fibroblast activation protein (FAP) may be an important risk factor for neurovascular metastasis in hepatocellular carcinoma. Analysis of The Cancer Genome Atlas (TCGA) database showed that FAP mRNA was highly expressed in most human tumor tissues. The HPA database then verified that FAP was highly expressed in tumor tissues following protein translation. Survival analysis then showed that the level of FAP expression significantly affected the overall survival (OS), progress free interval (PFI), and disease specific survival (DSS) of patients with hepatocellular carcinoma.

电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
ELISA
- Immobilized FAP His Tag, Human (Cat. No. UA010165) at 1.0μg/mL (100μL/well) can bind FAP Recombinant Rabbit mAb (SDT-R132) (Cat. No. S0B2166) with EC50 of 2.15-3.34ng/mL.

引用文献(1)