热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 药物靶点和Fc受体 药物靶点

所有图片（1/3）

TMIGD2/CD28H Fc Chimera Protein, Human

价格 ￥3,000.00 （ 1-2周）
货号 UA010127
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    Q96BF3

  • 表达序列

    Leu23-Gly150, with C-terminal Human IgG1 Fc

  • 表达宿主

    HEK293

  • 分子量

    49-64kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.
背景介绍

  • Transmembrane and immunoglobulin domain containing 2 (TMIGD2）is new a immune checkpoint molecule of the B7:CD28 family which is a novel cell adhesion receptor that is expressed in various human organs and tissues, mainly in cells with epithelium and endothelium origins. TMIGD2 regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. TMIGD2 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of TMIGD2 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that TMIGD2 regulates capillary tube formation in vitro, and B16F melanoma cells engineered to express TMIGD2 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, TMIGD2, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)-containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2.Silencing of expression of SPIN90/WISH by siRNA in endothelial cells showed that SPIN90/WISH is required for capillary tube formation. These features of TMIGD2 suggest that TMIGD2 is a novel receptor that plays an important role in cell-cell interaction, cell migration, and angiogenesis.

  • 生物活性

    • Anti-His antibody Immobilized on CM5 Chip captured B7-H7/HHLA2 His Tag, Cynomolgus (Cat. No. UA010264), can bind TMIGD2/CD28H Fc Chimera, Human (Cat. No. UA010127) with an affinity constant of 73.34nM as determined in SPR assay.
    • Immobilized TMIGD2/CD28H Fc Chimera, Human (Cat. No. UA010127) at 2.0μg/mL (100μL/well) can bind B7-H7/HHLA2 His Tag, Cynomolgus (Cat. No. UA010264) with EC50 of 1.74-1.76μg/ml.

  • 电泳

    • 1μg (R: reducing conditions, N: non-reducing conditions).

评论(0)